Imaging:Article Title: Dynamic fibroblast–immune interactions shape recovery after brain injury
Article Snippet: Whole slide scanning (40×) was performed on an Aperio AT2 (Leica Biosystems). .. Primary antibodies used for mouse imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-αSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRβ (APB5, Invitrogen 14-1402-82, 1:500), rabbit anti-NG2 (Millipore Sigma ab5320, 1:500), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-collagen 1 (Southern Biotech 1310-01, 1:200 or 1:500), rabbit anti-collagen 6α1 (Novus Biologicals NB120-6588, 1:200), rat anti-periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), Syrian hamster-anti-CD3ε (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-IBA1 (Aif3, Fujifilm Wako 019-19741, 1:200–1:1,000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1,000), mouse anti-E-cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti-cCasp3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). .. For marmoset imaging, rabbit anti-COL6 (Abcam ab6588, 1:500) was used.
Article Title: Dynamic fibroblast-immune interactions shape wound healing after brain injury
Article Snippet: .. Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti- α SMA (Abcam ab5694, 1:300) rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), rabbit anti-Collagen 6 α 1 (Novus Biologicals NB120-6588, 1:200), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3 ε (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000). .. For marmoset imaging, rabbit anti-COL6 (Abcam ab6588, 1:500) was used.
Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
Article Title: Lung adventitial fibroblasts support type 2 Tregs to shape the response to influenza infection
Article Snippet: Sections were then mounted and imaged with a Leica SP8 confocal microscope. .. Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), rat anti-ER-TR7 (Novus Biologicals NG100-64932), goat anti-Pi16 (R&D Systems AF4929), rabbit anti-proSP-C (Millipore AB3786), goat anti-IL-33 (R&D Systems AF3626), and rat anti-EpCAM (BD Pharmingen 552370). .. Directly conjugated antibodies were also used including mouse anti-αSMA AF488 (eBioscience 53-9760-82), anti-αSMA AF405 (R&D Systems IC1420V025), rat anti-CD45 BV421 (BioLegend 103134), rat anti Lyve1 ef660 (eBioscience 50-0443-82) and rat anti-CD45 (BioLegend 103124).
other:
Immunostaining:Article Title: Galectin-3 is dispensable for thoracic aortic aneurysmal formation and rupture in mice
Article Snippet: .. Immunostaining for Gal-3 and other cell-surface markers was performed using the following antibodies: rabbit anti-Gal-3 antibodies (1 μg/mL; Abcam, ab76245), rabbit anti-α-SMC actin antibodies for smooth muscle cells (1 mg/mL; Abcam, ab5694), rabbit anti-CD68 for macrophages (0.1 mg/mL; Cell Signaling, 97778), rabbit anti-CD31 for endothelial cells (1 μg/mL; Abcam, ab281583), and rat anti-ER-TR7 for fibroblasts (2 μg/mL; Novus Biologicals, NB100-64932). .. Male and female Gal-3 WT and KO mice (3-4 weeks old) were randomly assigned to receive either normal drinking water (vehicle control) or water containing BAPN (0.1%, 0.3%, and 0.5% wt/vol; TCI Chemicals, A0796) for a period of 4 weeks., , Fresh drinking water with and without BAPN was replaced twice per week and monitored daily for any morbidity.
Chromatin Immunoprecipitation:Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
Flow Cytometry:Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
Magnetic Resonance Imaging:Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
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